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KMID : 0545120170270071257
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 7 p.1257 ~ p.1265
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Sea Buckthorn (Hippophae rhamnoides L.) Leaf Extracts Protect Neuronal PC-12 Cells from Oxidative Stress
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Cho Chi Heung
Jang Ho-Lim
Lee Mi-Gi
Kang Hee
Heo Ho-Jin
Kim Dae-Ok
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Abstract
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The present study was carried out to investigate the antioxidative and neuroprotective effects of sea buckthorn (Hippophae rhamnoides L.) leaves (SBL) harvested at different times. Reversedphase high-performance liquid chromatography analysis revealed five major phenolic compounds: ellagic acid, gallic acid, isorhamnetin, kaempferol, and quercetin. SBL harvested in August had the highest total phenolic and flavonoid contents and antioxidant capacity. Treatment of neuronal PC-12 cells with the ethyl acetate fraction of SBL harvested in August increased their viability and membrane integrity and reduced intracellular oxidative stress in a dose-dependent manner. The relative populations of both early and late apoptotic PC-12 cells were decreased by treatment with the SBL ethyl acetate fraction, based on flow cytometry analysis using annexin V-FITC/PI staining. These findings suggest that SBL can serve as a good source of antioxidants and medicinal agents that attenuate oxidative stress.
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KEYWORD
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Antioxidant capacity, apoptosis, flow cytometry, high-performance liquid chromatography, total phenolics, total flavonoids
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